Difference between revisions of "Part:BBa K523024"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K523024 short</partinfo> | <partinfo>BBa_K523024 short</partinfo> | ||
− | Cex exoglucanase from ''Cellulomonas fimi'' fused to CenA endoglucanase from the same species. | + | Cex exoglucanase from ''Cellulomonas fimi'' fused to CenA endoglucanase from the same species. The part contains, in order: |
+ | |||
+ | * The GH10 exoglucanase domain | ||
+ | * A (possibly inactivated) cellulose binding domain | ||
+ | * The GH6 endoglucanase domain | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 12:13, 28 October 2011
cex + cenA fusion (cxnA)
Cex exoglucanase from Cellulomonas fimi fused to CenA endoglucanase from the same species. The part contains, in order:
- The GH10 exoglucanase domain
- A (possibly inactivated) cellulose binding domain
- The GH6 endoglucanase domain
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 524
Illegal NotI site found at 2222 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2135
Illegal XhoI site found at 1633
Illegal XhoI site found at 1882 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 157
Illegal NgoMIV site found at 530
Illegal NgoMIV site found at 1032
Illegal NgoMIV site found at 1413
Illegal NgoMIV site found at 2338 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 577
Illegal SapI.rc site found at 660