Difference between revisions of "Part:BBa K649401"

Revision as of 09:25, 27 October 2011

Arg box

This part contains the Arg Box. The Arg Box is the arginine operator capable of binding the arginine repressor.

Urea concentration detected in bacterial samples on pSB3K3
This work is done by Natsuki Kubo.

As a way to derepress arginine biosynthesis we introduced Arg box on our part BBa_K649401 in addition to rocF gene on our part BBa_K649301. In this case, more urea was produced compared that when only rocF gene were introduced. These results show that Arg boxes are effectively derepressing arginine production by deactivating arginine repressor.

For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/data#1. our work in Tokyo_Tech 2011 wiki.]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 3
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 [[Image:RocF and Arg box.png|thumb|center|500px|Urea concentration detected in bacterial samples on pSB3K3<br />
-This work is done by Natsuki Kubo.]]
+This work is done by Natsuki Kubo.]]<br />
+As a way to derepress arginine biosynthesis we introduced Arg box on our part [https://parts.igem.org/Part:BBa_K649401 BBa_K649401] in addition to <i>rocF</i> gene on our part [https://parts.igem.org/Part:BBa_K649301 BBa_K649301]. In this case, more urea was produced compared that when only <i>rocF</i> gene were introduced. These results show that Arg boxes are effectively derepressing arginine production by deactivating arginine repressor.
 For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/data#1. our work in Tokyo_Tech 2011 wiki.]