Difference between revisions of "Part:BBa K606061"

(References)
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===References===
 
===References===
 
<biblio>
 
<biblio>
#1 pmid=9669336
+
#1 pmid=9669336 Mol Gen Genet. 1998 Jun;258(5):538-45.
 
</biblio>
 
</biblio>
  

Revision as of 20:50, 25 October 2011

SpoVG Ribosome Binding Site (RBS) for B. subtilis

SpoVG Ribosome Binding Site (RBS) for B. subtilis.

This is a strong ribosome binding site from suibtilis. This part is a copy of the part from Imperial college 2008, BBa_K143021, but we have synthetized it de-novo, using primer matching technique, and submitted it to the registry.

This parts also works fine in E. coli.

Usage and Biology

This part can be use to express proteins in both B. subtilis and E. coli, and leads to a strong expression of the protein. The original sequence come from the reference following reference [1]

References

<biblio>

  1. 1 pmid=9669336 Mol Gen Genet. 1998 Jun;258(5):538-45.

</biblio>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]