Difference between revisions of "Part:BBa K606025:Experience"
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'''Microscopy of double transformed pDAG470 / Biobricked YFP:TetR in <i>E. Coli</i>:''' | '''Microscopy of double transformed pDAG470 / Biobricked YFP:TetR in <i>E. Coli</i>:''' | ||
| − | <p>To characterize this part properly, we took pictures of different strains containing YFP:TetR alone and both TetO array (from pDAG479, D.Lane) and expression YFP:TetR system (BBa_K606027).</p> | + | <p>To characterize this part properly, we took pictures of different strains containing YFP:TetR alone and both TetO array (from pDAG479, D.Lane) and expression YFP:TetR system (<partinfo>BBa_K606027</partinfo>).</p> |
<center> | <center> | ||
Latest revision as of 16:28, 24 October 2011
Characterization: Biobricked YFP:TetR
Parts construction:
Origins of YFP:TetR are from pFX234 of D. Lane (Toulouse 2 University).
Cloning plan of YFP:TetR construction
We characterize it with TetO Array from pDAG479 plasmid of F-X Barre and D. Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004).
Microscopy of double transformed pDAG470 / Biobricked YFP:TetR in E. Coli:
To characterize this part properly, we took pictures of different strains containing YFP:TetR alone and both TetO array (from pDAG479, D.Lane) and expression YFP:TetR system (BBa_K606027).
The TetR-YFP construct which (emitter part) occasionally shows gross aggregated YFP.
After observing the cells carrying both the TetO and YFP:TetR constructs, we can obviously distinguish glowing dots in some cells. They reflect the behavior we expected.
More information on : TetO Array characterization (BBa_K606026) and iGEM Paris Bettencourt 2011 wiki [http://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion]
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