Difference between revisions of "Part:BBa K606026:Design"
(Undo revision 126245 by Kyauy (Talk))
 
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
Andrew Wright, Dave Lane, François-Xavier Barre, pFX234 plasmid.
+
Andrew Wright, Dave Lane, François-Xavier Barre, pDAG479 plasmid.
  
 
===References===
 
===References===
 
Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms, G. Scott Gordon et al, Cell, 1997<br>
 
Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms, G. Scott Gordon et al, Cell, 1997<br>
 
Kinetics of plasmid segregation in Escherichia coli, G. Scott Gordon et al, Molecular Microbiology, 2004
 
Kinetics of plasmid segregation in Escherichia coli, G. Scott Gordon et al, Molecular Microbiology, 2004

Latest revision as of 21:46, 21 October 2011

tetO Array


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2093
    Illegal BamHI site found at 95
    Illegal BamHI site found at 601
    Illegal BamHI site found at 1171
    Illegal BamHI site found at 1752
    Illegal BamHI site found at 1960
    Illegal BamHI site found at 2577
    Illegal BamHI site found at 2727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 117
    Illegal BsaI site found at 735
    Illegal BsaI site found at 793
    Illegal BsaI.rc site found at 428
    Illegal SapI site found at 1179
    Illegal SapI site found at 3528


Design Notes

Reduced sequence to avoid restriction site.

Source

Andrew Wright, Dave Lane, François-Xavier Barre, pDAG479 plasmid.

References

Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms, G. Scott Gordon et al, Cell, 1997
Kinetics of plasmid segregation in Escherichia coli, G. Scott Gordon et al, Molecular Microbiology, 2004