Difference between revisions of "Part:BBa K606024:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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==Characterization: Biobricked YFP:TetR==
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how you used this part and how it worked out.
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'''Parts construction''':<br>
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Origins of YFP:TetR are from pFX234 of D. Lane (Toulouse 2 University).
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<center><img src="https://static.igem.org/mediawiki/2011/c/cb/YFPtetR10.jpg">
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<p>Cloning plan of YFP:TetR construction</center></p>
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</html>
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<br><br>We characterize it with TetO Array from pDAG479 plasmid of F-X Barre and D. Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004).<br><br>
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'''Microscopy of double transformed pDAG470 / Biobricked YFP:TetR in <i>E. Coli</i>:'''
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<p>To characterize this part properly, we took pictures of different strains containing YFP:TetR alone and both TetO array (from pDAG479, D.Lane) and expression YFP:TetR system (BBa_K606027).</p>
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<center>
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{| border="1" class="wikitable" style="text-align: center;" align="center"
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|+ Biobricked YFP:TetR only
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|[[Image:yfptetrbb_trans.jpg|350px|thumb|center|Biobricked expression YFP:tetR system in E. coli.]]
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|[[Image:yfptetrbb_Fluo2.jpg|350px|thumb|center|Biobricked expression YFP:tetR system in E. coli.]]
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|}
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{| border="1" class="wikitable" style="text-align: center;" align="center"
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|+ Biobricked YFP:TetR and TetO Array
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|[[Image:yfptetrbb_teto_trans.jpg|350px|thumb|center|Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.]]
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|[[Image:yfptetrbb_teto__Fluo2.jpg|350px|thumb|center|Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.]]
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|}
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</center>
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The TetR-YFP construct which (emitter part) occasionally shows gross aggregated YFP. <br>
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After observing the cells carrying both the TetO and YFP:TetR constructs, we can obviously distinguish glowing dots in some cells. They reflect the behavior we expected.
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===Applications of BBa_K606024===
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More information on : TetO Array characterization (<partinfo>BBa_K606026</partinfo>) and iGEM Paris Bettencourt 2011 wiki [http://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 16:27, 24 October 2011

Characterization: Biobricked YFP:TetR

Parts construction:
Origins of YFP:TetR are from pFX234 of D. Lane (Toulouse 2 University).

Cloning plan of YFP:TetR construction



We characterize it with TetO Array from pDAG479 plasmid of F-X Barre and D. Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004).

Microscopy of double transformed pDAG470 / Biobricked YFP:TetR in E. Coli:

To characterize this part properly, we took pictures of different strains containing YFP:TetR alone and both TetO array (from pDAG479, D.Lane) and expression YFP:TetR system (BBa_K606027).

Biobricked YFP:TetR only
Biobricked expression YFP:tetR system in E. coli.
Biobricked expression YFP:tetR system in E. coli.
Biobricked YFP:TetR and TetO Array
Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.
Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.

The TetR-YFP construct which (emitter part) occasionally shows gross aggregated YFP.
After observing the cells carrying both the TetO and YFP:TetR constructs, we can obviously distinguish glowing dots in some cells. They reflect the behavior we expected.


More information on : TetO Array characterization (BBa_K606026) and iGEM Paris Bettencourt 2011 wiki [http://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion]

User Reviews

UNIQba5ebb35c0eef010-partinfo-00000002-QINU UNIQba5ebb35c0eef010-partinfo-00000003-QINU