Difference between revisions of "Part:BBa K559000:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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| + | ===Characterizations of BBa_K559000=== | ||
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| + | '''Size Confirmation of the Biobrick''' | ||
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| + | [[Image:Gel_BBa_K559000.png]] | ||
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| + | The above figure shows the size check of our biobrick. Lane 1 represents 100 bp DNA ladder (NEB, cat. N3231L) , lane 3 represents our biobrick halorhodopsin gene. It matched the theoretical size of 640bp. | ||
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===Applications of BBa_K559000=== | ===Applications of BBa_K559000=== | ||
Latest revision as of 22:13, 14 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Characterizations of BBa_K559000
Size Confirmation of the Biobrick
The above figure shows the size check of our biobrick. Lane 1 represents 100 bp DNA ladder (NEB, cat. N3231L) , lane 3 represents our biobrick halorhodopsin gene. It matched the theoretical size of 640bp.
Applications of BBa_K559000
We have used this biobrick as a basic unit to construct a functional unit BBa_K559010.
More characterizations on the regulated protein expression, chloride absorption condition and optimization were shown in BBa_K559010.
User Reviews
UNIQ3d347505deecc16b-partinfo-00000002-QINU
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BBa_K559000, integrated into the halorhodopsin complete system generator BBa_K559010, express Maxmized halorhodopsin membrane protein when supplemented with 0.1mM IPTG. Characterization of the IPTG addition to get maximum expression can be view in [1] |
UNIQ3d347505deecc16b-partinfo-00000006-QINU