Difference between revisions of "Part:BBa K622005:Experience"

(→‎Characterization of σ54 promoter activity)
(→‎Characterization of σ54 promoter activity)
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We performed the function check of &sigma;<sup>54</sup> promoter in the genome of ''E. coli''.
 
We performed the function check of &sigma;<sup>54</sup> promoter in the genome of ''E. coli''.
  
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By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are repressed to less than one-fifth of that in 0.0 % glutamine.
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By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). The activity in 0.0 % glutamine was defined as a standard and those in 0.2 and 2.0 % glutamine were  Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are repressed to less than one-fifth of that in 0.0 % glutamine.
  
 
[[Image:Kyoto-sigma54-1.png|400px|thumb|center|Fig.1: The &sigma;<sup>54</sup> promoter activity in the different glutamine concentration. The activity in 0.0 % glutamine was defined as a standard and the activities in 0.2 and 2.0 % were the relative values.]]
 
[[Image:Kyoto-sigma54-1.png|400px|thumb|center|Fig.1: The &sigma;<sup>54</sup> promoter activity in the different glutamine concentration. The activity in 0.0 % glutamine was defined as a standard and the activities in 0.2 and 2.0 % were the relative values.]]

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Characterization of σ54 promoter activity

We performed the function check of σ54 promoter in the genome of E. coli.

By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). The activity in 0.0 % glutamine was defined as a standard and those in 0.2 and 2.0 % glutamine were Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are repressed to less than one-fifth of that in 0.0 % glutamine.

Fig.1: The σ54 promoter activity in the different glutamine concentration. The activity in 0.0 % glutamine was defined as a standard and the activities in 0.2 and 2.0 % were the relative values.

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