Difference between revisions of "Part:BBa K622005:Experience"

(→‎Characterization of σ54 promoter activity)
(→‎Characterization of σ54 promoter activity)
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===Characterization of &sigma;<sup>54</sup> promoter activity===
 
===Characterization of &sigma;<sup>54</sup> promoter activity===
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The function check of &sigma;<sup>54</sup> promoter in the genome of ''E. coli'' was performed with different glutamine concentration: 0.0%, 0.2%, and 2.0%.
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We performed the function check of &sigma;<sup>54</sup> promoter in the genome of ''E. coli''.
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The
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By measuring the amount of mRNA, the promoter activities in the different glutamine concentration were measured (Fig.1).
  
 
[[Image:Kyoto-sigma54-1.png|400px|thumb|center|Fig.1: &sigma;<sup>54</sup> promoter activity.]]
 
[[Image:Kyoto-sigma54-1.png|400px|thumb|center|Fig.1: &sigma;<sup>54</sup> promoter activity.]]

Revision as of 10:09, 14 October 2011

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Characterization of σ54 promoter activity

We performed the function check of σ54 promoter in the genome of E. coli.

By measuring the amount of mRNA, the promoter activities in the different glutamine concentration were measured (Fig.1).

Fig.1: σ54 promoter activity.

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