Difference between revisions of "Part:BBa K676011:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Performed PCR to cloned out the GBS from the pSC101 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.
+
Performed PCR to clone out the GBS from the pSC101 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.
  
 
===Source===
 
===Source===

Revision as of 02:13, 10 October 2011

Gyrase Binding Site from pSC101


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Performed PCR to clone out the GBS from the pSC101 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.

Source

pSC101 Plasmid DNA - 4580 to 4864 bp

References

Mark Oram , Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003)A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu , pSC101 and pBR322 Strong gyrase Sites ; the role of DNA sequence in modulating gyrase supercoiling and biological activity ; Molecular Microbiology 50(1).333-347