Difference between revisions of "Part:BBa K632002"
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Revision as of 23:30, 9 October 2011
INP-Silicatein
The silicatein gene has been successfully fused to an existent ice nucleation protein (BBa_K265009). Gel electrophoresis and DNA sequencing both suggest the fusion product is the correct size, frame, and orientation. Silicatein functional assay was performed to verify the activity of silicatein, and the results indicate the silicatein is enzymatically active.
Usage and Biology
The INP-Silicatein fusion protein is a chimeric protein with the outer membrane associated domain (N-terminal domain) of ice nucleation protein fused to silicatein which is capable of polymerizing silicic acid monomers. The 2011 University of Minnesota iGEM team generated the following data regarding this part:
