Difference between revisions of "Part:BBa K676002:Design"

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===Design Notes===
 
===Design Notes===
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The mNARK promoter used was from the UCL 2009 Stress Light device
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First Stage: Performed 3A assembly between mNark promoter ([[Part:BBa_K239006]]) and RBS ([[Part:BBa_B0034]]) and pSB1K3 plasmid backbone.
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Performed 3A assembly between YFP.LVA ([[Part:BBa_E0032]]) and terminator ([[Part:BBa_B0012]]) and pSB1T3 plasmid backbone.
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Second Stage: Performed 3A assembly between mNark promoter/RBS composite part ([[Part:BBa_K239006]] and YFP.LVA/Ter composite part ([[Part:BBa_B0034]]) and pSB1C3 plasmid backbone.
  
 
[[Image:Stress_Lights_2.0_Experiment.jpg]]
 
[[Image:Stress_Lights_2.0_Experiment.jpg]]

Revision as of 21:04, 9 October 2011

mNARK with YFP.LVA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

First Stage: Performed 3A assembly between mNark promoter (Part:BBa_K239006) and RBS (Part:BBa_B0034) and pSB1K3 plasmid backbone. Performed 3A assembly between YFP.LVA (Part:BBa_E0032) and terminator (Part:BBa_B0012) and pSB1T3 plasmid backbone.

Second Stage: Performed 3A assembly between mNark promoter/RBS composite part (Part:BBa_K239006 and YFP.LVA/Ter composite part (Part:BBa_B0034) and pSB1C3 plasmid backbone.

Stress Lights 2.0 Experiment.jpg

Source

mNarK promoter is designed to have better affinity to activated Fnr than the NarK promoter (BBa_K239005) and hence also stronger activity under anaerobic conditions. It is also shorter than the NarK promoter (BBa_K239005).

References