Difference between revisions of "Part:BBa K624057"

Revision as of 12:20, 9 October 2011

pYMB essentials + RBS(Pmsp3) + LLO

pYMB (BBa_K624004)is a shuttle vector for manipulating and transforming gene into AMB-1.

The ribosome binding site is from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012).

Listeriolysin O (LLO)is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2567
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 898
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 pYMB essentials + RBS(Pmsp3) + LLO
-pYMB  is a shuttle vector for manipulating and transforming gene into AMB-1.
+pYMB ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624004 BBa_K624004])is a shuttle vector for manipulating and transforming gene into AMB-1.
-RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter
-of AMB-1 .
-LLO (Listeriolysin O) can make the bacteria be able to
-escape into the cytosol without damaging the plasma
-membrane, and grow intracellularly.
-which help protect the AMB-1 from extracellular immune system.
-The ribosome binding site is from Pmsp3.(BBa_K624012).
-The ribosome binding site is from Pmsp3.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012]).
+The ribosome binding site is from Pmsp3, the strongest promoter
+of AMB-1.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012]).
+Listeriolysin O (LLO)is a hemolysin produced by the bacterium ''Listeria monocytogenes'', a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed ''L. monocytogenes'' cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.
 <!-- Add more about the biology of this part here