Difference between revisions of "Part:BBa K624055"

 
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Pmsp1 + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP
 
Pmsp1 + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP
  
The Pmsp1 is the second strongest promoter  
+
The Pmsp1 is the second strongest promoter on AMB-1.  
on AMB-1.  
+
 
RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter  
+
RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012])
of AMB-1
+
 
tetR serves as a switch,which can turn on the downstream gene
+
TetR serves as a switch, which can turn on the downstream gene
when Tetracycline is present ;and vice versa.
+
when tetracycline is present; and vice versa.
 +
 
 
GFP serves as a reporter to show if the construct work or not.
 
GFP serves as a reporter to show if the construct work or not.
All of the ribosome binding sites are RBS from Pmsp3.(BBa_K624012).
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:27, 9 October 2011

pYMB essentials + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP

Pmsp1 + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP

The Pmsp1 is the second strongest promoter on AMB-1.

RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012)

TetR serves as a switch, which can turn on the downstream gene when tetracycline is present; and vice versa.

GFP serves as a reporter to show if the construct work or not.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2561