Difference between revisions of "Part:BBa K539712"
 
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<br> Our team has mutated the function of RE site within the original '' ilvD''.
 
<br> Our team has mutated the function of RE site within the original '' ilvD''.
 
<br>https://static.igem.org/mediawiki/parts/d/d7/Ilvd.png
 
<br>https://static.igem.org/mediawiki/parts/d/d7/Ilvd.png
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 09:39, 8 October 2011

ilvD (dihydroxyacid dehydratase)

This is the coding sequence of ilvD form Escherichia coli str. K-12 substr. MG1655. It encodes dihydroxyacid dehydratase which is part of isobutanol biosynthesis pathway. Following is the isobutanol biosythesis pathway.
Pathway%28small%29.jpg
Our team has mutated the function of RE site within the original ilvD.
Ilvd.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1507
    Illegal BsaI site found at 1764