Difference between revisions of "Part:BBa K539742:Design"
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===Design Notes=== | ===Design Notes=== | ||
The gene Kivd is cloned from Escherichia coli str. K-12 substr(MG1655) by our team, NCTU-Formosa. We also modify the RE site in the coding sequencing. | The gene Kivd is cloned from Escherichia coli str. K-12 substr(MG1655) by our team, NCTU-Formosa. We also modify the RE site in the coding sequencing. | ||
| + | <br>https://static.igem.org/mediawiki/parts/7/7d/Alss.png | ||
===Source=== | ===Source=== | ||
Latest revision as of 06:40, 7 October 2011
TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_B0015)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 658
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene Kivd is cloned from Escherichia coli str. K-12 substr(MG1655) by our team, NCTU-Formosa. We also modify the RE site in the coding sequencing.
Source
It is cloned form Lactococcus lactis subsp. lactis KF147