Difference between revisions of "Part:BBa K572100"
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Revision as of 04:08, 6 October 2011
Light based screening plasmid backbone - pSB1Pc
The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2139
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2145 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2139
Illegal XhoI site found at 1033
Illegal XhoI site found at 2015 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2139
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2139
Plasmid lacks a suffix.
Illegal XbaI site found at 2154
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media.