Difference between revisions of "Part:BBa K629003"
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Note: Because the promoter is Plac here, the expression has a background, which means control also expresses some cheZ without induction of IPTG. | Note: Because the promoter is Plac here, the expression has a background, which means control also expresses some cheZ without induction of IPTG. | ||
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Revision as of 02:17, 6 October 2011
cheZ, chemotaxis regulator, protein phosphatase for CheY
Background
Polar localization is dependent on CheA-short. CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex. Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).
Expression in E. Coli
Before we started our functional test of CheZ, we have utilized western blot to determine whether there are enough soluble proteins in E. Coli. With 6×His tag on pET-32a, the result shows that CheZ has more expression with induction of IPTG.
Note: Because the promoter is Plac here, the expression has a background, which means control also expresses some cheZ without induction of IPTG.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]