Difference between revisions of "Part:BBa K594009:Design"

(→‎Design Notes)
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===Source===
 
===Source===
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We got the Rhizobium etli CFN42 from Nanjing Agriculture University, and then cloned the raiI gene from Rhizobium etli CFN42 with primers designed by the part design tool in the website [ginkgobioworks.com]. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers.
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The promoter of raiI is from the Rhizobium etli CFN42.
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===References===
 
===References===

Revision as of 00:29, 6 October 2011

the promoter of raiI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 444
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 447


Design Notes

The Rhizobium etli CFN42 is also a mode organism in Rhizobia. We looked for the papers related to the Quorum Sensing systems in Rhizobium etli CFN42, and then found the specific sequence in NCBI and asked the Rhizobium etli CFN42 from Nanjing Agricultural University. The raiI/R system have no threats to biosafety.

Source

We got the Rhizobium etli CFN42 from Nanjing Agriculture University, and then cloned the raiI gene from Rhizobium etli CFN42 with primers designed by the part design tool in the website [ginkgobioworks.com]. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers.

References