Difference between revisions of "Part:BBa K624057"

Revision as of 11:20, 9 October 2011

pYMB essentials + RBS(Pmsp3) + LLO

pYMB is a shuttle vector for manipulating and transforming gene into AMB-1 RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1 LLO (Listeriolysin O) can make the bacteria be able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. which help protect the AMB-1 from extracellular immune system. The ribosome binding site is from Pmsp3.(BBa_K624012).

The ribosome binding site is from Pmsp3.(BBa_K624012).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2567
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 898
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 pYMB essentials + RBS(Pmsp3) + LLO
+pYMB  is a shuttle vector for manipulating and transforming gene into AMB-1
+RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter
+of AMB-1
+LLO (Listeriolysin O) can make the bacteria be able to
+escape into the cytosol without damaging the plasma
+membrane, and grow intracellularly.
+which help protect the AMB-1 from extracellular immune system.
+The ribosome binding site is from Pmsp3.(BBa_K624012).
 The ribosome binding site is from Pmsp3.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012]).