Difference between revisions of "Part:BBa K624053"
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mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP | mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP | ||
− | This part is composed of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K624041 BBa_K624041] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K624002 BBa_K624002], both of two are submitted by 2011 NYMU-Taipei iGEM Team. The former subpart is in reverse order. We chose not to assembly BBa_K624041 in the downstream of BBa_K624002. If so, unwanted leakiness may happen. The construct was inserted in pUC19 and then was transformed into E. coli. | + | This part is composed of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K624041 BBa_K624041] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K624002 BBa_K624002], both of two are submitted by 2011 NYMU-Taipei iGEM Team. The former subpart is in reverse order. We chose not to assembly BBa_K624041 in the downstream of BBa_K624002. If so, unwanted leakiness may happen. The construct was inserted in pUC19 and then was transformed into E. coli(BL21). |
[[Image:E.coli_minC_NYMU.jpg]] | [[Image:E.coli_minC_NYMU.jpg]] |
Latest revision as of 03:22, 6 October 2011
mcherry+minC+RBS+tetO+pT7 + pT7+RBS+tetR+GFP
mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP
This part is composed of BBa_K624041 and BBa_K624002, both of two are submitted by 2011 NYMU-Taipei iGEM Team. The former subpart is in reverse order. We chose not to assembly BBa_K624041 in the downstream of BBa_K624002. If so, unwanted leakiness may happen. The construct was inserted in pUC19 and then was transformed into E. coli(BL21).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 803
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 803
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 803
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 803
Illegal AgeI site found at 681 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2898