Difference between revisions of "Part:BBa K559000"
Line 2: Line 2:
 
<partinfo>BBa_K559000 short</partinfo>
 
<partinfo>BBa_K559000 short</partinfo>
  
−
This Halorhodopsin gene allow the its membrane protein to be expressed. It regulates the chloride ion pumped from extracellular to intercellular space, with the rate being controlled by light intensity and quality as well as light exposure time.
+
-Halorhodopsin, a light-driven ion pump originated from Halobacterium, employs light to transport chloride ions into cells uni-directionally against osmolality.
 +
-Halorhodopsin has the fascinated property to transform solar energy, the most abundant energy in the world, into intracellular chloride ion level.  
 +
 
 +
This figure illustrates the molecular mechanism of Halorhodopsin gene on chloride ion absorption, in a controlled manner under light illumination.
 +
 
 +
[[Image:HR_mach.png]]
 +
 
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
Line 15: Line 21:
  
 
===Usage and Biology===
 
===Usage and Biology===
−
The figure illustrates the molecular mechanism of halorhodopsin gene on chloride ion absoption, in a controlled manner under light illumination.
+
We have use this biobrick to construct a functional halorhodopsin system unit <partinfo>BBa_K559010 parameters</partinfo>
  
−
[[Image:HR_mach.png]]
 
  
 
1. Essen, L.O. Halorhodopsin: light-driven ion pumping madesimple? ''Current opinion in structural biology'' 12, 516–522(2002)
 
1. Essen, L.O. Halorhodopsin: light-driven ion pumping madesimple? ''Current opinion in structural biology'' 12, 516–522(2002)

Revision as of 21:11, 14 October 2011

Halorhodopsin

-Halorhodopsin, a light-driven ion pump originated from Halobacterium, employs light to transport chloride ions into cells uni-directionally against osmolality. -Halorhodopsin has the fascinated property to transform solar energy, the most abundant energy in the world, into intracellular chloride ion level.

This figure illustrates the molecular mechanism of Halorhodopsin gene on chloride ion absorption, in a controlled manner under light illumination.

HR mach.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 280
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 292
    Illegal AgeI site found at 18
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 228



Usage and Biology

We have use this biobrick to construct a functional halorhodopsin system unit
biology-NA-
device_type-NA-
function-NA-
protein-NA-


1. Essen, L.O. Halorhodopsin: light-driven ion pumping madesimple? Current opinion in structural biology 12, 516–522(2002)