Difference between revisions of "Part:BBa K594001:Design"

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===Design Notes===
 
===Design Notes===
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The Sinorhizobium meliloti 1021 is a mode organism in Rhizobia. so We got the S. meliloti 1021 from Nanjing Agriculture University easily, and then cloned the sinI gene from S. meliloti 1021 with primers designed by the part design tool in the website [ginkgobioworks.com].
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The Sinorhizobium meliloti 1021 is a mode organism in Rhizobia. We looked for the papers related to the Quorum Sensing systems in S. meliloti Rm 1021, and then found the specific sequence in NCBI and asked the S. meliloti Rm 1021 strain from Nanjing Agricultural University.
  
 
===Source===
 
===Source===

Revision as of 21:44, 5 October 2011

a luxI homologous AHL synthetase from Sinorhizobium meliloti 1021


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Sinorhizobium meliloti 1021 is a mode organism in Rhizobia. We looked for the papers related to the Quorum Sensing systems in S. meliloti Rm 1021, and then found the specific sequence in NCBI and asked the S. meliloti Rm 1021 strain from Nanjing Agricultural University.

Source

We got the S. meliloti 1021 from Nanjing Agriculture University, and then cloned the sinI gene from S. meliloti 1021 with primers designed by the part design tool in the website [ginkgobioworks.com]. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers.

References