Difference between revisions of "Part:BBa K624054"
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<partinfo>BBa_K624054 short</partinfo> | <partinfo>BBa_K624054 short</partinfo> | ||
mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP | mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP | ||
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+ | This part is composed of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K624045 BBa_K624045] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K624055 BBa_K624055], both of two are submitted by 2011 NYMU-Taipei iGEM Team. The former subpart is in reverse order. We chose not to assembly BBa_K624045 in the downstream of BBa_K624055. If so, unwanted leakiness may happen. The construct was inserted into pYMB([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624027 BBa_K624027]) and then was electropored into AMB-1([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624032 BBa_K624032]). | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 23:09, 5 October 2011
mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP
mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP
This part is composed of BBa_K624045 and BBa_K624055, both of two are submitted by 2011 NYMU-Taipei iGEM Team. The former subpart is in reverse order. We chose not to assembly BBa_K624045 in the downstream of BBa_K624055. If so, unwanted leakiness may happen. The construct was inserted into pYMB(BBa_K624027) and then was electropored into AMB-1(BBa_K624032).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 842
Illegal PstI site found at 1622 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 842
Illegal PstI site found at 1622 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 842
Illegal PstI site found at 1622 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 842
Illegal PstI site found at 1622
Illegal NgoMIV site found at 2599
Illegal AgeI site found at 681 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4262