Difference between revisions of "Part:BBa K544001"
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Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors | Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors | ||
Revision as of 18:48, 5 October 2011
Tetracycline Repressor with Consitutive Promoter BBa_J23103
A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 65
Illegal SpeI site found at 37 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 65
Illegal SpeI site found at 37 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 65
Illegal SpeI site found at 37 - 1000COMPATIBLE WITH RFC[1000]
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Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors (The data collected are considered only when difference between values with the P-value <0.05)
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| Our team leader is demonstrating the pipetting techniques to the high school students. |
Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors (The data collected are considered only when difference between values with the P-value <0.05) [EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]