Difference between revisions of "Part:BBa K649301"

Revision as of 04:02, 27 October 2011

Ptrc-RBS-rocF

Arginase is coded with Ptrc and RBS in this part.

rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.

You should NOT ligate this part into high-copy vector.
In our test, when this part was ligated into pSB1C3, E. coli DH5α didn't glow even after 24 hours incubation.

Urea concentration in growth media 1 hour after IPTG induction.
This work is done by Natsuki Kubo.

Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655 (argR +). This part produced urea more efficiently in strain JD24293 (argR -).

For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/method our work in Tokyo_Tech 2011 wiki.]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 230
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 In our test, when this part was ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5&alpha; didn't glow even after 24 hours incubation.<br />
-[[Image:BBa K649301 graph(2).png|thumb|center|500px|Urea concentration in growth media 1 hour after IPTG induction.<br />
+[[Image:RocF.png|thumb|center|500px|Urea concentration in growth media 1 hour after IPTG induction.<br />
 This work is done by Natsuki Kubo.]]