Difference between revisions of "Part:BBa K539691:Experience"
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And to realize whether ''Alss,ilvC and ilvD'' have any impact to the production of isobutanol, we compare the circuit which contain ''Alss,ilvC and ilvD'' genes with the one with only a single ''kivd'' gene in it. | And to realize whether ''Alss,ilvC and ilvD'' have any impact to the production of isobutanol, we compare the circuit which contain ''Alss,ilvC and ilvD'' genes with the one with only a single ''kivd'' gene in it. | ||
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===User Reviews=== | ===User Reviews=== | ||
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Revision as of 16:18, 5 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K539691
Experience by 2011 iGEM NCTU_FORMOSA
Figure.1 Firstly , we ligase B0030 with ilvC.
Figure.2 At the sametime , we ligase B0030 with ilvD.
Figure.3 Meanwhile , we ligase B0032 with Alss.
Figure.4 To make sure we obtain the correct part , colony PCR is needed.
Figure.5 Secondly , we ligase Plac with B0032+Alss
Figure.6 Thirdly , we ligase Plac+B0032+Alss with B0030+ilvC
Figure.7 At the same time , we ligase B0030+ilvD with RNA thermometer+tetR+double terminator
Figure.8 Finally , we ligase the whole circuit:promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator
We cotransformed Part:BBa_K539691 and Part:BBa_K539742 into DH5α, so the expression of Alss, ilvC, ilvD and kivd are under the control of temperature.
And to realize whether Alss,ilvC and ilvD have any impact to the production of isobutanol, we compare the circuit which contain Alss,ilvC and ilvD genes with the one with only a single kivd gene in it.
User Reviews
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