Difference between revisions of "Part:BBa K533006:Experience"
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After Pro-rich mCherry expression, significant color change can be observed (bacteria cells turned cherry red as a result of mCherry expression) | After Pro-rich mCherry expression, significant color change can be observed (bacteria cells turned cherry red as a result of mCherry expression) | ||
| − | [[ | + | [[Image:Thuexp_cherryexp.png]] |
''From left to right, IPTG concentration: 0, 0.1mM, 0.5mM, 1mM. All induced at 18 centigrade.'' | ''From left to right, IPTG concentration: 0, 0.1mM, 0.5mM, 1mM. All induced at 18 centigrade.'' | ||
Revision as of 16:11, 5 October 2011
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Applications of BBa_K533006
This is the expression device of mCherry.
When IPTG is added, strong expression of mCherry can be detected. However, mCherry cannot be properly folded above 20 centigrade, as shown by our experimental results.
| Group | 1 | 2 |
|---|---|---|
| Volume | 3ml | |
| IPTG(mM) | 0.1 | |
| 0.5 | ||
| 1.0 | ||
| Temperature | 30oC | 18oC |
| Duration | 12h | 12h |
| Result | Media remained yellowish. E. coli white |
Media turned purple. E. coli cherry pink |
After Pro-rich mCherry expression, significant color change can be observed (bacteria cells turned cherry red as a result of mCherry expression)
From left to right, IPTG concentration: 0, 0.1mM, 0.5mM, 1mM. All induced at 18 centigrade.
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