Difference between revisions of "Part:BBa K581011"
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===Design Notes=== | ===Design Notes=== | ||
| − | ▪ A silent mutation (G->A) at base 1116 was made to remove | + | ▪ A silent mutation (G->A) at base 1116 was made to remove XbaI site |
▪ cqsA was amplified by PCR | ▪ cqsA was amplified by PCR | ||
Revision as of 15:47, 5 October 2011
pBAD-rbs-cqsA-terminator
A generator of CAI-1 behind pBAD promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 708 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
▪ A silent mutation (G->A) at base 1116 was made to remove XbaI site
▪ cqsA was amplified by PCR
Source
▪ We received the genome DNA of Vibrio cholera from Chinese Center for Disease Control and Prevention
▪ Although our system includes several genes from Vibrio cholera, these genes are not directly involved with virulence or antibiotic resistance in any case.