Difference between revisions of "Part:BBa K543015"
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Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. Although not perfectly repressed GFP expression, we confirmed Anti-killing Device is working. <br>
 
Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. Although not perfectly repressed GFP expression, we confirmed Anti-killing Device is working. <br>
 
Need to more experience to get quantitative date.  
 
Need to more experience to get quantitative date.  
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[[Image:killing 2.jpg]]
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[[Image:Tokyo_Metropolitan_Killing2.png|right]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:42, 5 October 2011

TetR generator (J23100-B0031-R0040-B0015)

TetR generator (J23100-B0031-R0040-B0015).

Repress expression of GFP by Anti-killing device
We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence.
Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. Although not perfectly repressed GFP expression, we confirmed Anti-killing Device is working.
Need to more experience to get quantitative date.

File:Tokyo Metropolitan Killing2.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]