Difference between revisions of "Part:BBa K543015"
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TetR generator (J23100-B0031-R0040-B0015).
 
TetR generator (J23100-B0031-R0040-B0015).
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<font size="3"><b>Repress expression of GFP by Anti-killing device</b></font><br>
 
<font size="3"><b>Repress expression of GFP by Anti-killing device</b></font><br>
 
<table>
 
<table>
 
<tr><td width="500">
 
<tr><td width="500">
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We confirm whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522:Ptet-B0034-GFP-B0015) and observe its florescence. Figure2 is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. Although not perfectly repressed GFP expression, we confirmed Anti-killing Device is working. Figure5 is difference of GFP florescence by comparing with absorbance of 525nm.
+
We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence. <br>
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For perfectly repressed expression cording under Ptet, RBS is needed to be more strong(ex.B0034).In addition, we should do more experience to get quantitative date.
+
Figure2 is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. Although not perfectly repressed GFP expression, we confirmed Anti-killing Device is working.<br>
 +
Need more experience to get quantitative date.<br>
  
 
</td><td width="300"><img src="https://static.igem.org/mediawiki/2011/c/cd/Tokyo_Metropolitan_Killing2.png" width="300"><br><div align="center">Figure5: repress expression of GFP by Anti-killing device</div></td></tr>
 
</td><td width="300"><img src="https://static.igem.org/mediawiki/2011/c/cd/Tokyo_Metropolitan_Killing2.png" width="300"><br><div align="center">Figure5: repress expression of GFP by Anti-killing device</div></td></tr>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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quantitative research
 
 
<!-- -->
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 15:33, 5 October 2011

TetR generator (J23100-B0031-R0040-B0015)

TetR generator (J23100-B0031-R0040-B0015).

Repress expression of GFP by Anti-killing device

We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence.
Figure2 is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. Although not perfectly repressed GFP expression, we confirmed Anti-killing Device is working.
Need more experience to get quantitative date.

<img src="Tokyo_Metropolitan_Killing2.png" width="300">
Figure5: repress expression of GFP by Anti-killing device

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]