Difference between revisions of "Part:BBa J23109:Experience"
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<I>iGEM HKU 2011</I> | <I>iGEM HKU 2011</I> | ||
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Revision as of 14:07, 5 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J23109
BBa_K190025 (Planning) constitutive promoter with GVP cluster
BBa_K190031 (Planning) constitutive promoter with fMT
User Reviews
UNIQd17ee6f1c4924f74-partinfo-00000000-QINU
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iGEM Groningen 2009 |
We used a number of the constitutive promoter family members for testing our biobricks. The constitutive promoters show the expected level of fluorescence when transformed into E. coli TOP10 cells. Placing parts behind the promoters turned out to be relatively straight forward. We used this part in combination with several biobricks for building our constructs e.g. BBa_I750016 and BBa_K190028 were placed behind the promoters. |
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iGEM HKU 2011 |
To start characterizing the promoters, we have performed the red florescence intensity measurements for our selected plasmid in the E.Coli MG1655 strain. The data collected is shown below. It is found that promoter J23106 can lead to a higher expression since the fluorescence intensity per OD600 is the highest, while J23103, J23109, J23116 have relative low expression and fluorescence. As our selected promoters have different strength, thus our team is able to use them to fine tune the protein expression. UNIQd17ee6f1c4924f74-partinfo-00000005-QINU |
