Difference between revisions of "Part:BBa K524003:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The <i>nadE</i> gene in E. Coli chromosome is terminated by terminated by downstream extragenic sites. The extragenic sties were replaced with double terminator B0015 in our construct. | + | The <i>nadE</i> gene in <i>E. Coli</i> chromosome is terminated by terminated by downstream extragenic sites. The extragenic sties were replaced with double terminator B0015 in our construct. |
===Source=== | ===Source=== |
Revision as of 13:52, 5 October 2011
nadE gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The nadE gene in E. Coli chromosome is terminated by terminated by downstream extragenic sites. The extragenic sties were replaced with double terminator B0015 in our construct.
Source
The nadE gene was cloned out from the genome of E. coli strain BL21(DE3).
References
Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism