Difference between revisions of "Part:BBa K519010"
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SmtA is a metallothionein that can bind to heavy metal ions such as Cd(II).  This SmtA is cloned from <i>Synechococcus</i> sp. PCC7942, a cyanobacterial strain. SmtA is a type II metallothionein with a reduced cys content.It was reported that marine cyanobacterium that does not originally harbor a homologous gene, when genetically engineered to express SmtA, could tolerate medium containing Cd(II), Zn(II) and Cu(II) (Sode <i>et al</I>., 1998).
 
SmtA is a metallothionein that can bind to heavy metal ions such as Cd(II).  This SmtA is cloned from <i>Synechococcus</i> sp. PCC7942, a cyanobacterial strain. SmtA is a type II metallothionein with a reduced cys content.It was reported that marine cyanobacterium that does not originally harbor a homologous gene, when genetically engineered to express SmtA, could tolerate medium containing Cd(II), Zn(II) and Cu(II) (Sode <i>et al</I>., 1998).
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https://static.igem.org/mediawiki/2011/d/dd/Metallothionein2.jpg
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The data above, taken from a paper (Sode <i>et al.</i>, 1988), shows that a cyanobacterial strain was able to tolerate medium of high Cd(II) concentration when it harbored the <i>smtA</i> gene. 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 01:13, 6 October 2011

SmtA

SmtA is a metallothionein that can bind to heavy metal ions such as Cd(II). This SmtA is cloned from Synechococcus sp. PCC7942, a cyanobacterial strain. SmtA is a type II metallothionein with a reduced cys content.It was reported that marine cyanobacterium that does not originally harbor a homologous gene, when genetically engineered to express SmtA, could tolerate medium containing Cd(II), Zn(II) and Cu(II) (Sode et al., 1998).


Metallothionein2.jpg

The data above, taken from a paper (Sode et al., 1988), shows that a cyanobacterial strain was able to tolerate medium of high Cd(II) concentration when it harbored the smtA gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 93
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 93
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 93
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 93
    Illegal AgeI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]