Difference between revisions of "Part:BBa I719005:Experience"

Revision as of 13:51, 19 October 2020

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I719005

SUSTech_Shenzhen

Protein anti-CRISPR-associated 1 (Aca1) is expressed by T7 promoter, Aca1 will subsequently inhibit the anti-CRISPR promoter, so the gene expression downstream anti-CRISPR will be inhibited until Navitoclax (a compound that can inhibit Aca1 found by SUSTech_Shenzhen) is added artificially. Without the inhibition from Aca1, the expression of genes downstream anti-CRISPR promoter will increase significantly. Therefore, protein expression can be regulated artificially.

T7 promoter successfully worked in our composite part BBa_K3423005.

User Reviews

UNIQ5625c06f2b145cee-partinfo-00000000-QINU

•••••

Red_Dave

Macquarie_Australia (2011) used this T7 promoter to successfully express hemeoxygenase and create part BBa_K646003.

UNIQ5625c06f2b145cee-partinfo-00000002-QINU

@@ Line 4: / Line 4: @@
 ===Applications of BBa_I719005===
+===SUSTech_Shenzhen===
+Protein anti-CRISPR-associated 1 (Aca1) is expressed by T7 promoter, Aca1 will subsequently inhibit the anti-CRISPR promoter, so the gene expression downstream anti-CRISPR will be inhibited until Navitoclax (a compound that can inhibit Aca1 found by SUSTech_Shenzhen) is added artificially. Without the inhibition from Aca1, the expression of genes downstream anti-CRISPR promoter will increase significantly. Therefore, protein expression can be regulated artificially.
+T7 promoter successfully worked in our composite part BBa_K3423005.
 ===User Reviews===