Difference between revisions of "Part:BBa K649000"

Revision as of 00:59, 5 October 2011

LasR and 3OC12HSL regulated promoter.

In the presence of 3OC12HSL lasR can bind to the operator of this promoter and activate the transcription of downstream gene, whereas in the absence of 3OC12HSL lasR can't bind to the operator and can't activate the transcription.

To characterize this promoter, we constructed the reporter part composed of BBa_K649000 and BBa_J54103. To know detailed infomation, please click here.

We improved previous lasI promoter(BBa_J64010).our assay of BBa_J64010

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-To characterize this promoter, we constructed the reporter part composed of BBa_K649000 and BBa_J54103. If you want to see detailed infomation, please click [https://parts.igem.org/Part:BBa_K649001 here.]
+To characterize this promoter, we constructed the reporter part composed of BBa_K649000 and BBa_J54103. To know detailed infomation, please click [https://parts.igem.org/Part:BBa_K649001 here].
 We improved previous lasI promoter(BBa_J64010).[https://parts.igem.org/Part:BBa_J64010:Experience our assay of BBa_J64010]
-For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#3. our work in Tokyo_Tech 2011 wiki]