Difference between revisions of "Part:BBa K649101"
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<partinfo>BBa_K649101 short</partinfo>
 
<partinfo>BBa_K649101 short</partinfo>
  
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PlsrR and lsrR work correctly.
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LsrR is the repressor of the lsr operon and itself. LsrK phosphates Auto Inducer 2(AI-2) and phospate AI-2 relieves LsrR repression. This part contains lsrR and lsrK gene, on downstream of lsrR promoter. lsrR and lsrR promoter in this part work correctly, but lsrK has flame shift mutation and does not work properly.
  
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However, in this part,lsrK has mutation and does not work properly.
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LsrR is the repressor of the lsr operon and itself.
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Revision as of 05:05, 5 October 2011

PlsrR-lsrR

LsrR is the repressor of the lsr operon and itself. LsrK phosphates Auto Inducer 2(AI-2) and phospate AI-2 relieves LsrR repression. This part contains lsrR and lsrK gene, on downstream of lsrR promoter. lsrR and lsrR promoter in this part work correctly, but lsrK has flame shift mutation and does not work properly.



To confirm LsrR represses lsrA promoter, we constructed BBa_K649105. If you want to see detailed infomation, please click here.


For more information, see our work in [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. Tokyo_Tech 2011 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1725
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1399
  • 1000
    COMPATIBLE WITH RFC[1000]