Difference between revisions of "Part:BBa K646000"

Revision as of 00:24, 5 October 2011

Hemeoxygenase with RBS

Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.

Usage and Biology

When coupled to a promoter BioBrick such as T7 promoter (BBa_I719005 and BBa_I712074) to produce parts BBa_K646003 and BBa_K646004, and transformed into Bl21(DE3)Tuner E. coli cells, a green colouration (require induction with ALA and IPTG) is produced.

Liquid media containing IPTG and ALA, incubated for 24 hours at low temperature, spun down. Pellets are green as heme oxyganse was expressed using T7 promoter and degraded heme into biliverdin.

Sequence and Features