Difference between revisions of "Part:BBa K649202"
Notothenia (Talk | contribs) |
Notothenia (Talk | contribs) |
||
| Line 2: | Line 2: | ||
<partinfo>BBa_K649202 short</partinfo> | <partinfo>BBa_K649202 short</partinfo> | ||
| − | The lox sequences, lox71 and lox66, have 5 bp on the 5 and 3 ends changed, respectively. DNA segment flanked by lox71 and lox66 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied and proved to be working. | + | The lox sequences, lox71 and lox66, have 5 bp on the 5 and 3 ends changed, respectively. DNA segment flanked by lox71 and lox66 marks the point which the enzyme Cre will excise. This Part is expected to express GFP when the lox sites are excised and RFP when they are not. The Cre-mediated recombination of this BioBrick had been studied and proved to be working. |
[[Image:111001DK_1hr_D7166_111003.png|thumb|center|500px|Effect of Cre recombinase induced by arabinose.]] | [[Image:111001DK_1hr_D7166_111003.png|thumb|center|500px|Effect of Cre recombinase induced by arabinose.]] | ||
Revision as of 23:43, 3 October 2011
PlacIQ-lox71-rfp-lox66-gfp
The lox sequences, lox71 and lox66, have 5 bp on the 5 and 3 ends changed, respectively. DNA segment flanked by lox71 and lox66 marks the point which the enzyme Cre will excise. This Part is expected to express GFP when the lox sites are excised and RFP when they are not. The Cre-mediated recombination of this BioBrick had been studied and proved to be working.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1016
Illegal BamHI site found at 14 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 686
Illegal AgeI site found at 798 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1700