Difference between revisions of "Part:BBa K518004:Experience"
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We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but failed to do that. In the previous works (Chao, Lo, & Luo, 2000), the amount of L-Asp was determined by HPLC. We, however, were unable to use this machinery, so we tried to detect it through alternative method, ninhydrin reaction and ultraviolet-visible spectroscopy. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our result page]. | We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but failed to do that. In the previous works (Chao, Lo, & Luo, 2000), the amount of L-Asp was determined by HPLC. We, however, were unable to use this machinery, so we tried to detect it through alternative method, ninhydrin reaction and ultraviolet-visible spectroscopy. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our result page]. | ||
| − | Reference | + | =Reference= |
Chao, Y., Lo, T., & Luo, N. (2000). Selective production of L-aspartic acid and L-phenylalanine by coupling reactions of aspartase and aminotransferase in Escherichia coli. Enzyme Microb Technol, 27(1-2), 19-25. | Chao, Y., Lo, T., & Luo, N. (2000). Selective production of L-aspartic acid and L-phenylalanine by coupling reactions of aspartase and aminotransferase in Escherichia coli. Enzyme Microb Technol, 27(1-2), 19-25. | ||
Revision as of 19:13, 3 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K518004
User Reviews
UNIQ70e88d2e24714887-partinfo-00000000-QINU
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UT-Tokyo 2011 |
We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but failed to do that. In the previous works (Chao, Lo, & Luo, 2000), the amount of L-Asp was determined by HPLC. We, however, were unable to use this machinery, so we tried to detect it through alternative method, ninhydrin reaction and ultraviolet-visible spectroscopy. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our result page]. ReferenceChao, Y., Lo, T., & Luo, N. (2000). Selective production of L-aspartic acid and L-phenylalanine by coupling reactions of aspartase and aminotransferase in Escherichia coli. Enzyme Microb Technol, 27(1-2), 19-25.
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