Difference between revisions of "Part:BBa K649301"
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You should NOT ligate this part into high-copy vector.<br /> | You should NOT ligate this part into high-copy vector.<br /> | ||
In our test, when this part was ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5α didn't glow even after 24 hours incubation.<br /> | In our test, when this part was ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5α didn't glow even after 24 hours incubation.<br /> | ||
+ | |||
+ | [[Image:BBa K649301 graph.png|thumb|center|500px|introduction of <span style="font-style:italic;">rocF</span>(arginase coding gene) led to production of some urea. <br>This work was done by Natsuki Kubo and GoshiSugano.]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 18:32, 3 October 2011
Ptrc-RBS-rocF
Arginase is coded with Ptrc and RBS in this part.
rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.
You should NOT ligate this part into high-copy vector.
In our test, when this part was ligated into pSB1C3, E. coli DH5α didn't glow even after 24 hours incubation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 230
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]