Difference between revisions of "Part:BBa K629001"

 
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<partinfo>BBa_K629001 short</partinfo>
 
<partinfo>BBa_K629001 short</partinfo>
  
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Name: recAp
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The recA gene of Escherichia coli is involved in genetic recombination, in postreplication repair, and in a number of other cellular functions such as mutagenesis, phage induction, and cell division. RecA protein is related to SOS system-- DNA repair, which is activated by recA promoter. The active level of recA promoter is fluctuates from low basal levels to very high levels after treatments that damage DNA or stall DNA replication. In our project, recA promoter is used to detect single-chain DNA which result from ionizing radiation, x-ray, ultraviolet radiation and some kinds of drug. According to the charater, ionizing radiation can activate recA promoter and raise the level of gene expression which under recA promoter's regulation.
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+1: 2821841
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Sigma Factor: Sigma70 Sigmulon
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Distance from start of the gene: 50
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Sequence: cttgtggcaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttCaacagaacatattgactatc
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Evidence(s): [HIPP] Human inference of promoter position
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[TIM] Transcription initiation mapping
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Reference(s): [1] Pages V., et al., 2003
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:39, 5 October 2011

recAp, could be started with exposure to irradiation, UV, nalidixic acid

The recA gene of Escherichia coli is involved in genetic recombination, in postreplication repair, and in a number of other cellular functions such as mutagenesis, phage induction, and cell division. RecA protein is related to SOS system-- DNA repair, which is activated by recA promoter. The active level of recA promoter is fluctuates from low basal levels to very high levels after treatments that damage DNA or stall DNA replication. In our project, recA promoter is used to detect single-chain DNA which result from ionizing radiation, x-ray, ultraviolet radiation and some kinds of drug. According to the charater, ionizing radiation can activate recA promoter and raise the level of gene expression which under recA promoter's regulation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]