Difference between revisions of "Part:BBa K649200"
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lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise.
 
lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise.
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The Cre-mediated recombination of this BioBrick had been studied by both ''in vitro'' and ''in vivo'' assay, and proved to be working.
+
The Cre-mediated recombination of this BioBrick had been studied by ''in vitro'' assay, and proved to be working.
  
 
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Revision as of 09:14, 4 October 2011

PlacIQ-lox2272-gfp-lox2272

lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied by in vitro assay, and proved to be working.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 107
    Illegal BamHI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790