Difference between revisions of "Part:BBa K649104"

Revision as of 09:11, 3 October 2011

PlsrA-RBS-gfp

Median fluorescence intensity(MFI) of BBa_K649104 was much higher than that of promoterless-gfp(negative control).
This work is done by Takuya Tsubaki.

We measured the transcriptional activity of our lsrA promoter(BBa_K649100) by introducing a gfp gene downstream of the promoter. Its fluorescence intensity was much higher than that of promoterless-gfp(negative control), showing that our new lsrA promoter works. Moreover,fluorescence intensity of PlsrA(BBa_117002)-gfp was almost the same as a promoterless-gfp(negative control), showing that BBa_K117002 does not work properly.

We improved previous lsrA promoter(BBa_K117002).our assay of BBa_K117002

For more information, see our work in Tokyo_Tech 2011 wiki

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 770

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K649104 short</partinfo>
+[[Image:PlsrA_activity1.png|thumb|center|500px|Median fluorescence intensity(MFI) of BBa_K649104 was much higher than that of promoterless-gfp(negative control).<br>This work is done by Takuya Tsubaki.]]
-We measured the transcriptional activity of our lsrA promoter by introducing a gfp gene downstream of the promoter.  Its fluorescence intensity was much higher than that from a promoterless gfp negative control plasmid, showing that our new lsrA promoter works.
+We measured the transcriptional activity of our lsrA promoter(BBa_K649100) by introducing a gfp gene downstream of the promoter.  Its fluorescence intensity was much higher than that of promoterless-gfp(negative control), showing that our new lsrA promoter works. Moreover,fluorescence intensity of PlsrA(BBa_117002)-gfp was almost the same as a promoterless-gfp(negative control), showing that BBa_K117002 does not work properly.