Difference between revisions of "Part:BBa K649001"

Revision as of 08:53, 3 October 2011

GFP regulated by 3OC12-HSL and LasR

Fluorescence intensity of BBa_K649001 was increased by 3OC12-HSL induction.

Effect of 3OC12-HSL induction on fluorescence intensity
This work is done by Takuya Tsubaki.

Generally, in the presence of 3OC12-HSL, lasR activates lasI promoter and the transcription level of downstream gene increases, but in the absence of 3OC12-HSL, lasR can't activate lasI promoter. 2011 iGEM Tokyo-Tech characterized BBa_K649000 by means of using BBa_K649001 composed of BBa_K649000 and BBa_J54103.

We improved previous lasI promoter(BBa_J64010).our assay of BBa_J64010

For more information, see our work in Tokyo_Tech 2011 wiki

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 118
Illegal BamHI site found at 106
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 805

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−	[[Image:BBa K649001 ~~graph1~~.png\|thumb\|center\|500px\|Effect of 3OC12-HSL induction on fluorescence intensity<br>This work is done by Takuya Tsubaki.]]	+	[[Image:BBa K649001 graph2.png\|thumb\|center\|500px\|Effect of 3OC12-HSL induction on fluorescence intensity<br>This work is done by Takuya Tsubaki.]]