Difference between revisions of "Part:BBa K578004"
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===Confirmation of Part Length=== | ===Confirmation of Part Length=== | ||
| + | PCR of BBa_K578004 using Primers VF2 (BBa_G00100) and VFR (BBa_G00101) from pSB1C3. The part is 235bp plus 42bp for the Standard BioBrick prefix and suffix. Note: The primers add approximatellly 200bp to the BioBrick's size, thus BBa_K578004 should appear at ~477bp (Band appears larger than expected and requires sequence confirmation). | ||
[[Image: ASU_Bh_Leader.png|400px]] | [[Image: ASU_Bh_Leader.png|400px]] | ||
Latest revision as of 22:07, 5 October 2011
Bacillus halodurans C-125 CRISPR leader sequence
This is a putative leader sequence for the CRISPR III locus in Bacillus halodurans C-125.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Confirmation of Part Length
PCR of BBa_K578004 using Primers VF2 (BBa_G00100) and VFR (BBa_G00101) from pSB1C3. The part is 235bp plus 42bp for the Standard BioBrick prefix and suffix. Note: The primers add approximatellly 200bp to the BioBrick's size, thus BBa_K578004 should appear at ~477bp (Band appears larger than expected and requires sequence confirmation).
