Difference between revisions of "Part:BBa K578001"
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===Confirmation of Part Length=== | ===Confirmation of Part Length=== | ||
| + | PCR of BBa_K578001 using Primers VF2 (BBa_G00100) and VFR (BBa_G00101) from pSB1C3. The part is 106bp plus 42bp for the Standard BioBrick prefix and suffix. Note: The primers add approximatellly 200bp to the BioBrick's size (The primary band visualized is larger than expected an requires sequencing confirmation). | ||
[[Image: ASU_K12_Leader.png|400px]] | [[Image: ASU_K12_Leader.png|400px]] | ||
Revision as of 21:59, 5 October 2011
E. coli CRISPR I leader sequence
Usage and Biology
- This is the AT-rich leader region of the CRISPR I array in Escherichia coli str. K-12 substr. MG1655. "Identification and characterization of E. coli CRISPR-cas promoters and their silencing by H-NS" (Ü. Pul et al, 2010) characterized a single promoter (Pcrispr1) in this sequence. Transcription is silenced through H-NS binding to the leader sequence. Induction requires anti-silencing, either by protein-independent processes, DNA-binding proteins or other modulatory mechanisms not yet identified.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Confirmation of Part Length
PCR of BBa_K578001 using Primers VF2 (BBa_G00100) and VFR (BBa_G00101) from pSB1C3. The part is 106bp plus 42bp for the Standard BioBrick prefix and suffix. Note: The primers add approximatellly 200bp to the BioBrick's size (The primary band visualized is larger than expected an requires sequencing confirmation).
