Difference between revisions of "Part:BBa K557000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We | + | We obtained the sequence of CheZ of Top10 in NCBI (NP416395.1) and designed primers to acquire CheZ by PCR. |
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| + | forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGATGCAACCATCAATCAAACCTGCTGACGAGCAT-3' | ||
| + | reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTA-3' | ||
===Source=== | ===Source=== | ||
Revision as of 12:53, 2 October 2011
CheZ: phosphatase which dephosphorylates CheY-P and thus plays an important role in E.Coli chemotaxi
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We obtained the sequence of CheZ of Top10 in NCBI (NP416395.1) and designed primers to acquire CheZ by PCR.
forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGATGCAACCATCAATCAAACCTGCTGACGAGCAT-3'
reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTA-3'
Source
Escherichia coli, the strain has always been kept in the lab of USTC-IGEM
References
Shana Topp, Justin P. Gallivan (2006) Guiding Bacteria with Small Molecules and RNA. J.Am.Chem.Soc, 129,6870-6811