Difference between revisions of "Part:BBa K649105"
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<partinfo>BBa_K649105 short</partinfo> | <partinfo>BBa_K649105 short</partinfo> | ||
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[[Image:OD lsrR repression.png|thumb|center|400px|After four hours from OD590 reaching 0.15, we measured OD.<br>This work is done by Hiroki Yoshise.]] | [[Image:OD lsrR repression.png|thumb|center|400px|After four hours from OD590 reaching 0.15, we measured OD.<br>This work is done by Hiroki Yoshise.]] | ||
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− | We confirmed that LsrR represses lsrA promoter. | + | We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a LsrR gene downstream of PlsrA-gfp.By the LsrR repression, the fluorescence intensity decreased 3-fold. (Fig.6). This result shows that LsrR successfully repressed PlsrA. The working parts we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems. |
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For more information, see our work in Tokyo_Tech 2011 wiki | For more information, see our work in Tokyo_Tech 2011 wiki |
Revision as of 11:48, 2 October 2011
PlsrA-gfp-PlsrR-lsrR
We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a LsrR gene downstream of PlsrA-gfp.By the LsrR repression, the fluorescence intensity decreased 3-fold. (Fig.6). This result shows that LsrR successfully repressed PlsrA. The working parts we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.
For more information, see our work in Tokyo_Tech 2011 wiki
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2579
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2253
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 770