Difference between revisions of "Part:BBa K624019"

Revision as of 12:52, 4 October 2011

pYMB essentials+[13-R]+[Y-CHAMP]

pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, 13-R and Y-CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. This design is made in case EYFP fusion on N-terminal of mms13 has negative effects on its folding. But according to the opposite location of EYFP on CHAMP, this part is intended for a negative control, also testing the orientation of CHAMP on the effect of BRET between EYFP and rLuc.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 1809
Illegal NgoMIV site found at 1893
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2559

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K624019 short</partinfo>
-pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, 13-R and Y-CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. This design is made in case EYFP fusion on N-terminal of mms13 has negative effects on its folding.
+pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, 13-R and Y-CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. This design is made in case EYFP fusion on N-terminal of mms13 has negative effects on its folding. But according to the opposite location of EYFP on CHAMP, this part is intended for a negative control, also testing the orientation of CHAMP on the effect of BRET between EYFP and rLuc.